Optimization of Crude Oil Biodegradation of Fungi Isolated from Refinery Effluent Site using Response Surface Methodology

The activities involved in the production and exploration of crude oil has constantly polluted environment. This study investigated ability an indigenous fungus to utilize petroleum hydrocarbon. Response Surface Methodology was used optimize effects pH, microbial concentration (spores/ml), contact time (days) on removal efficiency refinery effluent. Monocillium sp. isolated for treatment effluent due its predominance contaminated soil. Twenty experimental runs were analyzed determine effect efficiency. From theexperimental results obtained, a maximum 98.42 % achieved at pH 6.5, 14 days, 3 spores/ml. obtained showed percentage sample increased with increase time. Optimization result 98.59 %, 13.96 6.85, 3.01 findings this revealed that is viable hydrocarbon degrader, can be bioremediation environments.
 Keywords: surface, optimization, bioremediation, hydrocarbon, efficiency,